Supporters of the Brisbane Valley Rail Trail are re-booting their campaign to get funding for the under-developed tourist attraction.

Campaign organiser Paul Heymans said the former LNP Queensland Government made a $3.5 million grant available specifically for Rail Trails from the Royalties to Regions Fund in the 2014 Budget.

"(At the time) 1183 supporters signed a Change.org Petition calling on the Somerset Regional Council 'To apply for grant funding to complete the Brisbane Valley Rail Trail'," Mr Heymans said.

"In January 2015, Somerset Regional Council responded positively by passing a resolution to apply for the funding subject to the terms and conditions."

Mr Heymans said the timing was unfortunate because by then the Queensland State elections were called and there was a change in Government.

"It is not yet known what policy the current Labor Queensland Government has towards the Brisbane Valley Rail Trail, so we're rebooting the campaign with a new Petition," he said.,

The Petition will be supported by the 'Real Brisbane Valley Rail Trail End-to-End Bike Ride' which will be done in 40km sections commencing on Saturday, May 16.

The Rail Trail follows the disused Brisbane Valley rail line from Ipswich to Yarraman but there is a missing link from Toogoolawah to Moore.

Mr Heymans is urging supporters of the trail to support the new Change.org Petition calling on the Queensland Government to fund completion of the Brisbane Valley Rail Trail.